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Gal-Gal pyelonephritis Escherichia coli pili linear immunogenic and antigenic epitopes

机译：Gal-Gal肾盂肾炎大肠杆菌菌毛线性免疫原性和抗原表位

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The linear immunogenic and antigenic structure of E. coli Gal-Gal pili from the recombinant strain HU 849 was investigated with nine synthetic peptides corresponding to regions of the pilus sequence predicted to contain hydrophilic beta-turns. Five peptides, as bovine serum albumin conjugates, were found by anti-HU 849 pilus serum and were thus designated "immunogenic epitopes." Peptides corresponding to R 25-38, R 38-50, and R 48-61 (which jointly comprise the single intramolecular disulfide loop), and R 103-116, were bound in low titer. A prominent immunogenic epitope was specified by a peptide corresponding to R 65- 75. Four peptides, as thyroglobulin conjugates, elicited antisera in rabbits that bound intact HU 849 pili. These were designated "antigenic epitopes." Two prominent antigenic epitopes were localized to peptides corresponding to R 5-12 and R 93-104, whereas peptides corresponding to R 65-75 and R 119-131 represented two minor antigenic epitopes. None of the peptide antisera bound Gal-Gal pili from heterologous strains except anti-R 93-104 and anti-R 5-12. In 8 of the 10 Gal-Gal-binding pyelonephritis isolates tested, anti-R 5-12 detected a protein with an apparent molecular weight of 18,000 co-migrating with several Gal-Gal pili. Anti-R 93-104 detected a corresponding protein in 4 of 8 fecal and 7 of 12 pyelonephritis Gal-Gal-binding isolates; however, it also bound apparently unrelated proteins of higher molecular weight.
机译：研究了来自重组菌株HU 849的大肠杆菌Gal-Gal菌毛的线性免疫原性和抗原结构，使用了九种合成肽进行了研究，这些肽对应于菌毛序列的区域，预计包含亲水性β-转角。通过抗HU 849菌毛血清发现了五个肽，作为牛血清白蛋白结合物，因此被称为“免疫原性表位”。对应于R 25-38，R 38-50和R 48-61（共同组成一个分子内二硫键的环）和R 103-116的肽以低滴度结合。突出的免疫原性表位由对应于R 65-75的肽指定。四种肽，如甲状腺球蛋白缀合物，在结合完整HU 849菌毛的兔中引发抗血清。这些被称为“抗原表位”。两个突出的抗原表位位于对应于R 5-12和R 93-104的肽上，而对应于R 65-75和R 119-131的肽代表两个较小的抗原表位。除抗R 93-104和抗R 5-12外，没有肽抗血清结合来自异源菌株的Gal-Gal菌毛。在测试的10个结合Gal-Gal的肾盂肾炎分离株中的8个中，抗R 5-12检测到一种表观分子量为18,000的蛋白质与几个Gal-Gal菌毛共同迁移。抗R 93-104在8个粪便中有4个和12个肾盂肾炎Gal-Gal结合分离物中检测了7种相应的蛋白质。然而，它也结合了显然无关的高分子量蛋白。

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